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Mutagenesis of Leuconostoc Mesenteroides and selection of Dextransucrase hyperproducing strains
Dextransucrase Dextran Leuconostoc mesenteroides
2010/1/6
Dextransucrase of Leuconostoc mesenteroides PTCC 1059 is an enzyme of industrial and medical interest that catalyzes the synthesis of a soluble dextran from sucrose. The mutant strains of Leuconostoc ...
应用PCR技术对先天性长QT综合征KCNQ1基因进行定点突变的研究PCR Site-Directed Mutagenesis of Long QT Syndrome KCNQ1 Gene in vitro
长QT综合征 KCNQ1 PCR 定点突变
2008/1/20
摘要
利用聚合酶链反应（PCR）技术对长QT综合征（LQTS）KCNQ1基因进行定点突变的研究。首先设计两对引物（包含预定的突变），通过3轮PCR扩增，扩增出含有所需突变位点的片段，然后将片段克隆入T载体中，通过酶切连接的方法将突变点引入到pIRES2-EGFP-KCNQ1中，随后用Effectene转染试剂介导转染HEK293细胞。结果在真核表达载体pIRES2-EGFP-KCNQ1基础上获得...
Effect of cysteine mutagenesis on human IgE reactivity of recombinant forms of the major rye grass pollen allergen Lol p 1
allergy grass pollen Lol p 1 mutagenesis rye grass
2010/11/24
Background: Hypersensitivity to the group 1 grass pollen allergens is a significant causative factor in the onset of symptoms for hay fever sufferers. To better understand the IgE reactivity of the gr...