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Analysis of integration sites of transgenic sheep generated by lentiviral vectors using next-generation sequencing technology
Gene transfer gene therapy gene transfer the original nucleus
2015/5/22
The development of new methods to carry out gene transfer has many benefits to several fields, such as gene therapy, agriculture and animal health. The newly established lentiviral vector systems fur...
Rapid Identification of Transgenic Cotton (Gossypium hirsutum L.) Plants by Loop-mediated Isothermal Amplification
Bt chitinase CryIA(b) detection loop-mediated isothermal amplification transgenic cotton
2014/2/25
In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was ext...
番茄转录调控因子基因Pti5植物表达载体的构建及其转化烟草的研究Construction of a Plant Expression Vector with Tomato Transcription Factor Gene Pti5 and Studies on Transgenic Tobaccos
Pti5-VP16基因 表达载体 转化 烟草
2007/12/31
摘要本实验构建了含有CaMV35S启动子控制下的Pti5-VP16基因的植物双元表达载体pBI121UCH1。通过根癌农杆菌叶盘转化法，将Pti5-VP16基因导入烟草SRI中，经卡那霉素筛选，获得了抗性植株。经PCR和Southern印迹分析，表明抗性植株中整合了Pti5-VP16基因，经抗病性鉴定转基因烟草植株的抗病性明显提高。
Abstract:The plasmid pBI121UCH1...
摘要通过长距离PCR从山羊基因组DNA分两段扩增山羊β乳球蛋白（β-lactoglobulin,BLG）基因，扩增出的两个片段分别克隆到T载体上，利用BLG基因序列自身存在的NarI单酶切位点进行拼接，获得了全长为7.2kb的山羊BLG基因克隆，并构建了它的真核表达载体，经酶切鉴定和序列分析证实了克隆的正确性。用线性化的BLG基因显微注射小鼠受精卵以建立转基因鼠，经PCR和Southern印迹分析...